No, it’s from cover art for his biography:
http://mayflowerhistory.com/bookstore-biographies
There is no known image made in his lifetime.
Interesting but we more than likely will never know exactly or remotely what he looked like which is unfortunate as is his various adventures and endeavors . Amazing how Bradford gets so much attention when it was the Hopkins family that survived the harsh winter months after lading in the new land now called America. I am a great granddaughter of Stephen’s(11th)